EZ Fast Zebrafish PCR Genotyping Kit
Automated Genomic DNA Extraction with
<10-second Hands-on Time per Sample
EZ Fast Zebrafish PCR Genotyping Kit is a new
generation of genotyping kits which is designed for rapid extraction and
amplification of genomic DNA from zebrafish tissues without DNA
purification. With automated one-solution extraction, extraction of genomic DNA
from animal tissue is as easy as pushing a start button. No termination buffer is needed. There is no
need for overnight digestion, Phenol/Chloroform extraction, and laborious DNA
purification. Neither there is a requirement for use of expensive DNA spin
columns. Most importantly of all, the consistent result from EZ Fast Zebrafish Genotyping
Kit is unmatched by any other DNA extraction-based genotyping methods available
in the market! The kit includes Tissue Lysis Solution A and B and 2X EZ PCR
Ready Master Mix. The 2x EZ PCR Master Mix containing Taq polymerase and dNTPs
is specially optimized for use with the extracted DNA. It also contains an
inert red dye that allows direct loading of PCR products onto agarose gels
without the addition of loading dye.
New Extraction Protocol
1) Add DNA Extraction Buffer to the Strip tube with a
tail sample.
2) Place the strip tube in a thermocycler and start the
extraction program
3) After 20-min, take out the strip tube and invert 5
times.
Extraction is done and genomic DNA is ready for PCR.
Features and Benefits
Automated extraction:
Push-button DNA extraction with <10-second hands-on time.
Fast Protocol:
Genomic DNA ready for PCR in 20 min. No overnight digestion
Multiplexing:
Strip tube for parallel processing
Easy Handling: One solution extraction. No termination
buffer
Significant savings:
No need to purify genomic DNA with expensive spin columns.
Consistent results:
Virtually 100% success rate!