The Tri-Color Prestained Protein Molecular Weight Marker is a three-color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green band at 25 kDa and one red band at 75 kDa when separated on SDS polyacrylamide gel using Tris-Glycine buffer). The Tri-Color Prestained Protein Molecular Weight Marker is designed for monitoring protein separated during SDS-PAGE, for verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The Tri-Color Prestained Protein Molecular Weight Marker is supplied in gel loading buffer and is in ready to use format. No need to heat, dilute, or add reducing agent before loading.
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
Apply more for thicker (>1.5 mm) or larger gel.