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EZ Tissue/Cell Total RNA Mini Plus Kit 
 
EZ Tissue/Cell Total RNA Mini Plus Kit is designed for purification of high quality total RNA from variety of mammalian tissues or cultured cells. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The contaminated genomic DNA is removed by DNase digestion. Any other impurities are removed by stringent washing steps. The purified RNA is eluted in RNase-Free Water. The kit eliminates the need for toxic organic extraction and time-consuming ethanol precipitation steps required in the other RNA purification methods. The purified RNA is virtually free of Genomic DNA contamination and is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
   
Features and Benefits

RNA Purity:

O.D260/2801.9

RNA Yield:

2-4 µg/mg tissue or 10-25 µg/1 x 10^6 cells

RNA Size:

>200 nucleotides

Spin Column Binding Capacity:

Up to 200 µg RNA

Maximal Starting Material:

Up to 30 mg of tissue or 1 x 10^7 cells

Elution Volume:

50 µl

 
Performance  
 
 
Kit Components
 

Components

R1004-50

R1004-250

RNA Lysis/Binding Buffer

30 ml

75 ml x 2

Wash Buffer I

47.5 ml

118.75 ml x 2

Wash Buffer II

10 ml

25 ml x 2

RNase-Free DNase I

1 tube

5 tubes

DNase Reconstitution Buffer 

0.5 ml

0.5 ml x 5

10 X DNase Digestion Buffer

0.6 ml

3 ml

RNase-Free Water

10 ml

20 ml

Pre-Clean Column Units

50

250

Spin Column Units

50

250

Collection Tubes (2 ml)

50

250

Collection Tubes (1.5 ml)

50

250

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Products (Total Items: 4)

R1004-50 EZ Tissue/Cell Total RNA Mini Plus Kit 50 preps $204.00
R1004-250 EZ Tissue/Cell Total RNA Mini Plus Kit 250 preps $799.00
R1002-50-pre Pre-Clean Spin Columns (50) for RNA Kits 50 $30.00
R1002-250-Pre Pre-Clean Spin Columns (250) for RNA Kits 250 $145.00

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