(0)  item(s) in your cart Total: $0
Tel: 1(800) 637-0262  Fax: 1(877) 693-4868
»Tissue/Tail PCR Genotyping
»SV Tissue/Tail Genotyping
»Eco-Direct Tissue/Tail Genotyping
»Blood/Cell PCR Genotyping
»Zebrafish PCR Genotyping
»Tissue/Tail DNA + PCR Kit
»High MW Tail DNA + PCR Kit
»Taq DNA Polymerase
»Turbo Taq DNA Polymerase
»HotStart Taq DNA Polymerase
»HF Taq DNA Polymerase
»Long Taq DNA Polymerase
»2X Taq Master Mix
»2X Taq Red Master Mix
»2X Turbo Taq Master Mix
»2X Turbo Taq Red Master Mix
»dNTP Mix
»Tissue/Tail DNA Isolation
»Blood/Cells DNA Isolation
»High MW Tissue DNA Isolation
»High MW Blood DNA Isolation
»High MW Tail DNA Isolation
»High MW Cell DNA Isolation
»High MW Bacterial DNA Isolation
»Plasmid Miniprep
»High Speed Plasmid Maxiprep
»Plasmid Maxiprep
»PCR Clean-Up
»Gel Extraction
»Tissue/cell RNA Mini Kit
»Tissue/cell RNA Mini Plus Kit
»RNA Clean-Up
»RNA Clean-Up Plus DNase Kit
»EZ Blood Total RNA Isolation
»Stabilized Blood RNA Isolation
»EZ RNA Storage Solution
»EZ MicroElute-10 DNA Clean-Up Kit
»EZ MicroElute-25 DNA Clean-Up Kit
»EZ DNA MW Markers
»EZ Protein MW Markers
»Water and Reagents
»Agar Plates
»Pipette Tips
»PCR Tubes
»Microcentrifuge Tubes
»Centrifuge Tubes
»Culture Tubes
»T-shape Spreaders
»Inoculating Needles
»Petri Dishes
»Freezer Boxes
»Plastic Freezer Boxes
»Cryogenic Vials
»Cell Culture Plates
»Cell Culture Flasks
»Cell Culture Chamber Slides
»Cell Culture Dishes
»Cell Scrapers and Lifters
»Serological Pipettes
»Cell Strainer
»Science Kits
 
EZ Tissue/Cell Total RNA Miniprep Kit
 
EZ Tissue/Cell Total RNA Mini Kit is designed for purification of high quality total RNA from variety of mammalian tissues or cultured cells. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The contaminated genomic DNA is removed by DNase digestion. Any other impurities are removed by stringent washing steps. The purified RNA is eluted in RNase-Free Water. The kit eliminates the need for toxic organic extraction and time-consuming ethanol precipitation steps required in the other RNA purification methods. The purified RNA is virtually free of Genomic DNA contamination and is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
  
Features and Benefits
 

RNA Purity:

O.D260/2801.9

RNA Yield:

2-4 g/mg tissue or 10-25 g/1 x 10^6 cells

RNA Size:

>200 nucleotides

Spin Column Binding Capacity:

Up to 200 g RNA

Maximal Starting Material:

Up to 30 mg of tissue or 1 x 10^7 cells

Elution Volume:

50 l

 
Performance

            Agarose gel electrophresis analysis of RNA isolated from different sources using EZ Tissue/Cell RNA Miniprep Kit
 
 
            Real time RT-PCR analysis of various amounts of RNA isolated from 3 different preps using EZ Tissue/Cell RNA Miniprep Kit.
 
 
Kit Components
 

Components

R1002-50

R1002-250

RNA Lysis/Binding Buffer

30 ml

75 ml x 2

Wash Buffer I

47.5 ml

118.75 ml x 2

Wash Buffer II

10 ml

25 ml x 2

RNase-Free Water

10 ml

20 ml

Pre-Clean Column Units

50

250

Spin Column Units

50

250

Collection Tubes (2 ml)

50

250

Collection Tubes (1.5 ml)

50

250

  
Products (Total Items: 4)

R1002-50 EZ Tissue/Cell Total RNA Mini Kit 50 preps $169.00
R1002-250 EZ Tissue/Cell Total RNA Mini Kit 250 preps $744.00
R1002-50-pre Pre-Clean Spin Columns (50) for RNA Kits 50 $30.00
R1002-250-Pre Pre-Clean Spin Columns (250) for RNA Kits 250 $145.00

Copyright © 2006 EZ BioResearch LLC LLC. All Rights Reserved.