Tissue/cell RNA Mini Kit

EZ Tissue/Cell Total RNA Miniprep Kit

EZ Tissue/Cell Total RNA Mini Kit is designed for purification of high quality total RNA from variety of mammalian tissues or cultured cells. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The contaminated genomic DNA is removed by DNase digestion. Any other impurities are removed by stringent washing steps. The purified RNA is eluted in RNase-Free Water. The kit eliminates the need for toxic organic extraction and time-consuming ethanol precipitation steps required in the other RNA purification methods. The purified RNA is virtually free of Genomic DNA contamination and is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.

Features and Benefits

RNA Purity:

O.D260/280=1.9

RNA Yield:

2-4 µg/mg tissue or 10-25 µg/1 x 10^6 cells

RNA Size:

>200 nucleotides

Spin Column Binding Capacity:

Up to 200 µg RNA

Maximal Starting Material:

Up to 30 mg of tissue or 1 x 10^7 cells

Elution Volume:

50 µl

Performance

Agarose gel electrophresis analysis of RNA isolated from different sources using EZ Tissue/Cell RNA Miniprep Kit

Real time RT-PCR analysis of various amounts of RNA isolated from 3 different preps using EZ Tissue/Cell RNA Miniprep Kit.

Kit Components

Components	R1002-50	R1002-250
RNA Lysis/Binding Buffer	30 ml	75 ml x 2
Wash Buffer I	47.5 ml	118.75 ml x 2
Wash Buffer II	10 ml	25 ml x 2
RNase-Free Water	10 ml	20 ml
Pre-Clean Column Units	50	250
Spin Column Units	50	250
Collection Tubes (2 ml)	50	250
Collection Tubes (1.5 ml)	50	250