EZ Tissue/Tail DNA Isolation Plus PCR Kit
EZ Tissue/Tail DNA Isolation Plus PCR Kit is designed for isolating genomic DNA from animal tissues including rodent tails and PCR genotyping of purified genomic DNA. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with Proteinase K in a specially formulated lysis buffer that allows protein denaturation. Residual RNA is removed by RNase digestion. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting. The 2X PCR Ready Mix included in the kit is specially formulated for genotyping. The enhanced formulation leads to less false negatives in genotyping. The 2X PCR Ready Mix contains an inert red dye that allows direct loading of PCR products onto agarose gels and eliminates the need for addition of loading dye.
Features and Benefits
|
DNA
Purity: |
Typical O.D 260/280
≥1.8,
free of RNA |
|
DNA
Size Range: |
>20
kb |
|
DNA
Yield: |
5-30 µg |
|
Spin
Column Binding Capacity: |
50 µg
DNA |
|
Elution
Volume: |
200 µl |
Performance
High Quality DNA purified from different tissues with EZ Tissue/Tail DNA Isolation Kit
M 1 2 3 4 5 6 7 8
Genomic DNA isolated from mouse tissues using EZ Tissue/Tail DNA Isolation Kit was digested with (Lanes 2,4,6,8) or without (Lanes 1,3,5,7) EcoRI and analyzed on a 0.8% agarose gel. Lanes 1,2: tail; Lanes 3,4: liver; Lanes 5,6: kidney; Lanes7,8: spleen. M: Lambda-HindIII ladder.
Typical DNA Yields Using EZ Tissue/Tail DNA Isolation Kit
|
Tissue Source |
Starting Amount |
DNA Yield (ug) |
|
Mouse Tail |
0.5-1.2 cm |
5-15 |
|
Liver |
25 mg |
15-30 |
|
Kidney |
25 mg |
10-25 |
|
Heart |
25 mg |
5-10 |
|
Lung |
25 mg |
5-10 |
|
Spleen |
10 mg |
5-25 |
|
Brain |
25 mg |
15-30 |
Kit Components
|
Components |
M1003-100 |
|
Tissue Lysis Buffer |
22 ml |
|
DNA Binding Buffer |
25 ml |
|
Wash Buffer I |
30 ml |
|
Wash Buffer II |
18 ml |
|
Elution Buffer |
50 ml |
|
Proteinase K |
2 ml |
|
RNaseA |
2 ml |
|
Spin Column Units |
100 |
|
Sample Collection Tubes |
100 |
|
2X PCR Ready Mix |
1 ml | |
