Tissue/Tail DNA Isolation

EZ Tissue/Tail DNA Isolation Kit

EZ Tissue/Tail DNA Isolation Kit is designed for isolating genomic DNA from animal tissues including rodent tails. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with Proteinase K in a specially formulated lysis buffer that allows protein denaturation. Residual RNA is removed by RNase digestion. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting.

Features and Benefits

DNA Purity:
DNA Size Range:
DNA Yield:
Spin Column Binding Capacity:
Elution Volume:

Typical O.D 260/280 > 1.8, free of RNA
> 20 kb
5-30 µg
50 µg
200 µl

Performance

High Quality DNA purified from different tissues with EZ Tissue/Tail DNA Isolation Kit

M 1 2 3 4 5 6 7 8

Genomic DNA isolated from mouse tissues using EZ Tissue/Tail DNA Isolation Kit was digested with (Lanes 2,4,6,8) or without (Lanes 1,3,5,7) EcoRI and analyzed on a 0.8% agarose gel. Lanes 1,2: tail; Lanes 3,4: liver; Lanes 5,6: kidney; Lanes7,8: spleen. M: Lambda-HindIII ladder.

Typical DNA Yields Using EZ Tissue/Tail DNA Isolation Kit

Tissue Source	Starting Amount	DNA Yield (ug)
Mouse Tail	0.5-1.2 cm	5-15
Liver	25 mg	15-30
Kidney	25 mg	10-25
Heart	25 mg	5-10
Lung	25 mg	5-10
Spleen	10 mg	5-25
Brain	25 mg	15-30

Kit Components

Components	M1003-50	M1003-250
Tissue Lysis Buffer	11 ml	50 ml
DNA Binding Buffer	13 ml	55 ml
Wash Buffer I	15 ml	62.5 ml
Wash Buffer II	9 ml	37.5 ml
Elution Buffer	25 ml	110 ml
Proteinase K	1 ml	5 ml
RNaseA	1 ml	5 ml
Spin Column Units	50	250
Sample Collection Tubes	50	250