EZ Tissue/Tail DNA Isolation Kit
EZ Tissue/Tail DNA Isolation Kit is designed for isolating genomic DNA from animal tissues including rodent tails. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with Proteinase K in a specially formulated lysis buffer that allows protein denaturation. Residual RNA is removed by RNase digestion. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting.
Features and Benefits
DNA Purity:
DNA Size Range:
DNA Yield:
Spin Column Binding Capacity:
Elution Volume:
| Typical O.D 260/280 > 1.8, free of RNA
> 20 kb
5-30 µg
50 µg
200 µl |
Performance
High Quality DNA purified from different tissues with EZ Tissue/Tail DNA Isolation Kit
M 1 2 3 4 5 6 7 8
Genomic DNA isolated from mouse tissues using EZ Tissue/Tail DNA Isolation Kit was digested with (Lanes 2,4,6,8) or without (Lanes 1,3,5,7) EcoRI and analyzed on a 0.8% agarose gel. Lanes 1,2: tail; Lanes 3,4: liver; Lanes 5,6: kidney; Lanes7,8: spleen. M: Lambda-HindIII ladder.
Typical DNA Yields Using EZ Tissue/Tail DNA Isolation Kit
|
Tissue Source |
Starting Amount |
DNA Yield (ug) |
|
Mouse Tail |
0.5-1.2 cm |
5-15 |
|
Liver |
25 mg |
15-30 |
|
Kidney |
25 mg |
10-25 |
|
Heart |
25 mg |
5-10 |
|
Lung |
25 mg |
5-10 |
|
Spleen |
10 mg |
5-25 |
|
Brain |
25 mg |
15-30 |
Kit Components
|
Components |
M1003-50 |
M1003-250 |
|
Tissue Lysis Buffer |
11 ml |
50 ml |
|
DNA Binding Buffer |
13 ml |
55 ml |
|
Wash Buffer I |
15 ml |
62.5 ml |
|
Wash Buffer II |
9 ml |
37.5 ml |
|
Elution Buffer |
25 ml |
110 ml |
|
Proteinase K |
1 ml |
5 ml |
|
RNaseA |
1 ml |
5 ml |
|
Spin Column Units |
50 |
250 |
|
Sample Collection Tubes |
50 |
250 |
|
