EZ Tissue/Tail DNA Isolation Plus PCR Kit is designed for isolating genomic DNA from animal tissues including rodent tails and PCR genotyping of purified genomic DNA. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with Proteinase K in a specially formulated lysis buffer that allows protein denaturation. Residual RNA is removed by RNase digestion. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting. The 2X PCR Ready Mix included in the kit is specially formulated for genotyping. The enhanced formulation leads to less false negatives in genotyping. The 2X PCR Ready Mix contains an inert red dye that allows direct loading of PCR products onto agarose gels and eliminates the need for addition of loading dye.

Features and Benefits

DNA purity: Typical O.D_260/280≥1.8, free of RNA

DNA size range: > 20 kb

DNA yield: 5-30 ug

Spin column binding capacity: 50 ug DNA

Elution volume: 200 ul

Performance

High Quality DNA purified from different tissues with EZ Tissue/Tail DNA Isolation Kit

M 1 2 3 4 5 6 7 8

Genomic DNA isolated from mouse tissues using EZ Tissue/Tail DNA Isolation Kit was digested with (Lanes 2,4,6,8) or without (Lanes 1,3,5,7) EcoRI and analyzed on a 0.8% agarose gel. Lanes 1,2: tail; Lanes 3,4: liver; Lanes 5,6: kidney; Lanes7,8: spleen. M: Lambda-HindIII ladder.

Typical DNA Yields Using EZ Tissue/Tail DNA Isolation Kit

Tissue Source	Starting Amount	DNA Yield (ug)
Mouse Tail	0.5-1.2 cm	5-15
Liver	25 mg	15-30
Kidney	25 mg	10-25
Heart	25 mg	5-10
Lung	25 mg	5-10
Spleen	10 mg	5-25
Brain	25 mg	15-30

Kit Components

Components	M1003-100	M1003-250
Tissue Lysis Buffer	22 ml	50 ml
DNA Binding Buffer	25 ml	55 ml
Wash Buffer I	30 ml	62.5 ml
Wash Buffer II	18 ml	37.5 ml
Elution Buffer	50 ml	110 ml
Proteinase K	2 ml	5 ml
RNaseA	2 ml	5 ml
Spin Column Units	100	250
Sample Collection Tubes	100	250
2X PCR Ready Mix	1 ml	2.5 ml

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G1003-100

Tissue/Tail DNA Isolation Plus PCR Kit

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Tissue/Tail DNA Isolation Plus PCR Kit

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