EZ RNA Clean-Up Plus DNase Kit
EZ RNA Clean Up Plus DNase Kit provides a rapid method to clean up RNA from crude RNA preps or after enzymatic reactions such as labeling reaction, RNA ligation, etc. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The crude RNA is mixed with RNA Lysis/Binding Buffer and the mixture is then loaded onto the silica membrane-based spin column. Genomic DNA contamination is removed with DNase treatment. Any other impurities are removed by subsequent washing steps. The purified RNA is eluted in RNase-Free Water. The purified RNA is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
Features and Benefits
|
RNA Purity: |
O.D260/280≥1.9 |
|
RNA Size: |
>200 nucleotides |
|
Spin Column Binding Capacity: |
Up to 200 µg RNA |
|
Elution Volume: |
50 µl |
Performance
Kit Components
|
Components |
R1003-50 |
R1003-250 |
|
RNA Lysis/Binding Buffer |
20 ml |
90 ml |
|
Wash Buffer |
8 ml x 2 |
25 ml x 3 |
|
RNase-Free Water |
10 ml |
20 ml |
|
RNase-Free DNase I |
1 tube |
5 tubes |
|
DNase Reconstitution Buffer |
0.5 ml |
0.5 ml x 5 |
|
10 X DNase Digestion Buffer |
0.6 ml |
3 ml |
|
Spin Column Units |
50 |
250 |
|
Collection Tubes (2 ml) |
50 |
250 |
|
Collection Tubes (1.5 ml) |
50 |
250 |
|
