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EZ RNA Clean-Up Plus DNase Kit 
 
EZ RNA Clean Up Plus DNase Kit provides a rapid method to clean up RNA from crude RNA preps or after enzymatic reactions such as labeling reaction, RNA ligation, etc. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The crude RNA is mixed with RNA Lysis/Binding Buffer and the mixture is then loaded onto the silica membrane-based spin column. Genomic DNA contamination is removed with DNase treatment. Any other impurities are removed by subsequent washing steps. The purified RNA is eluted in RNase-Free Water. The purified RNA is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
   
Features and Benefits
RNA Purity:
O.D260/2801.9
RNA Size:
>200 nucleotides
Spin Column Binding Capacity:
Up to 200 µg RNA
Elution Volume:
50 µl
 
Performance  
 
 
Kit Components 
 
Components
R1003-50
R1003-250
RNA Lysis/Binding Buffer
20 ml
90 ml
Wash Buffer
8 ml x 2
25 ml x 3
RNase-Free Water
10 ml
20 ml
RNase-Free DNase I
1 tube
5 tubes
DNase Reconstitution Buffer 
0.5 ml
0.5 ml x 5
10 X DNase Digestion Buffer
0.6 ml
3 ml
Spin Column Units
50
250
Collection Tubes (2 ml)
50
250
Collection Tubes (1.5 ml)
50
250

 

 
 
 
 
 
 
 
 
 
Products (Total Items: 2)

R1003-50 EZ RNA Clean Up Plus DNase Kit 50 preps $186.00
R1003-250 EZ RNA Clean Up Plus DNase Kit 250 preps $767.00

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