RNA Clean-Up Plus DNase Kit

EZ RNA Clean-Up Plus DNase Kit

EZ RNA Clean Up Plus DNase Kit provides a rapid method to clean up RNA from crude RNA preps or after enzymatic reactions such as labeling reaction, RNA ligation, etc. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The crude RNA is mixed with RNA Lysis/Binding Buffer and the mixture is then loaded onto the silica membrane-based spin column. Genomic DNA contamination is removed with DNase treatment. Any other impurities are removed by subsequent washing steps. The purified RNA is eluted in RNase-Free Water. The purified RNA is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.

Features and Benefits

RNA Purity:	O.D260/280=1.9
RNA Size:	>200 nucleotides
Spin Column Binding Capacity:	Up to 200 µg RNA
Elution Volume:	50 µl

Performance

Kit Components

Components	R1003-50	R1003-250
RNA Lysis/Binding Buffer	20 ml	90 ml
Wash Buffer	8 ml x 2	25 ml x 3
RNase-Free Water	10 ml	20 ml
RNase-Free DNase I	1 tube	5 tubes
DNase Reconstitution Buffer	0.5 ml	0.5 ml x 5
10 X DNase Digestion Buffer	0.6 ml	3 ml
Spin Column Units	50	250
Collection Tubes (2 ml)	50	250
Collection Tubes (1.5 ml)	50	250