Plasmid Miniprep

EZ Plasmid Miniprep Kit

EZ Plasmid Miniprep Kit is a silica membrane based spin column format kit for isolating high quality plasmid DNA (up to 30 µg) from E. coli host strain in 10 min. The principle of the kit is based on alkaline/SDS lysis of bacteria cells followed by selective adsorption of plasmid DNA on silica membrane in the presence of high salt. Contaminants are removed by wash buffer and the plasmid DNA is eluted in Elution Buffer. The purified DNA can be used for any downstream molecular biology applications.

Features and Benefits

DNA purity:

DNA yield:

Spin column binding capacity:

Elution volume:

Culture volume:

Easy:

Flexible:

O.D 260/280=1.8

Up to 30 µg

50 µg

50 µl

1-5 ml

10 min easy to follow protocol

Vacuum or spin format

Performance

Comparison of Plasmid Miniprep Kits from EZ BioResearch and Vendor Q

The binding capacity of the spin columns from EZ BioResearch is much higher than that of Vendor Q.

Kit Components

Components	M1000-50	M1000-250
Resuspension Buffer	14 ml	65 ml
Cell Lysis Buffer	14 ml	65 ml
Neutralization Buffer	19 ml	90 ml
Wash Buffer*	6 ml	25 ml
Elution Buffer	5 ml	20 ml
RNaseA	1 tube	1 tube
Spin Column Units	50	250
Sample Collection Tubes	50	250