EZ Plasmid Miniprep Kit
EZ Plasmid Miniprep Kit is a silica membrane based spin column format kit for isolating high quality plasmid DNA (up to 30 µg) from E. coli host strain in 10 min. The principle of the kit is based on alkaline/SDS lysis of bacteria cells followed by selective adsorption of plasmid DNA on silica membrane in the presence of high salt. Contaminants are removed by wash buffer and the plasmid DNA is eluted in Elution Buffer. The purified DNA can be used for any downstream molecular biology applications.
Features and Benefits
|
DNA purity:
DNA yield:
Spin column binding capacity:
Elution volume:
Culture volume:
Easy:
Flexible: |
O.D 260/280=1.8
Up to 30 µg
50 µg
50 µl
1-5 ml
10 min easy to follow protocol
Vacuum or spin format |
Performance
Comparison of Plasmid Miniprep Kits from EZ BioResearch and Vendor Q
The binding capacity of the spin columns from EZ BioResearch is much higher than that of Vendor Q.
Kit Components
|
Components |
M1000-50 |
M1000-250 |
|
Resuspension Buffer |
14 ml |
65 ml |
|
Cell Lysis Buffer |
14 ml |
65 ml |
|
Neutralization Buffer |
19 ml |
90 ml |
|
Wash Buffer* |
6 ml |
25 ml |
|
Elution Buffer |
5 ml |
20 ml |
|
RNaseA |
1 tube |
1 tube |
|
Spin Column Units |
50 |
250 |
|
Sample Collection Tubes |
50 |
250 | |
