PCR Clean-Up

EZ PCR Clean-Up Kit

EZ PCR Clean-Up Kit is a silica membrane based spin column format kit for purifying DNA fragments from PCR and other enzymatic reactions. The purified DNA that is free of primers, enzymes and unwanted reaction components can be used for any downstream molecular biology applications.

Features and Benefits

DNA Size Range:	Purify DNA fragments 100 bp-10 kb in size
Pure DNA:	Remove primers up to 40 bases completely
Recovery Rate:	90-98%
Spin Column Binding Capacity:	50 µg DNA
Fast:	5 min easy to follow protocol
Flexible:	Choice of spin or vacuum methods
Complete:	Kit contains all required tubes and reagents, ready to use

Performance

Complete removal of primers after PCR with EZ PCR Clean-Up Kit

1 2 3 4 5 6

-600 bp PCR product

-40 base primers

Lane 1: 1 Kb MW marker

Lane 2: PCR reaction before Purification

Lane 3 & 4: Purification with EZ PCR Clean-Up Kit

Lane 5 & 6: Purification with company Q PCR Clean-Up kit

Kit Components

Components	M1001-50	M1001-250
DNA Binding Buffer	28 ml	125 ml
Wash Buffer	6 ml	15 ml x 2
Elution Buffer	8 ml	20 ml
Spin Column Units	50	250
Sample Collection Tubes	50	250