EZ High Speed Plasmid Maxiprep Kit
30 min from pellet to DNA! The fastest and the easiest plasmid maxiprep kit available in the market!
EZ High Speed Plasmid Maxiprep Kit provides a simple and fast silica membrane based method to isolate high quality plasmid DNA from large recombinant E. coli culture. The principle of the kit is based on alkaline/SDS lysis of bacteria cells followed by selective adsorption of plasmid DNA on silica membrane in the presence of high salt. Contaminants are removed by wash buffers and the plasmid DNA is eluted in Elution Buffer. The EZ Filter Syringe included in the kit enables rapid clearance of lysate without centrifugation. Up to 1.2 mg of plasmid DNA can be isolated from 200 ml overnight culture grown in LB broth. The isolated plasmid DNA is predominantly in its super-coiled form. The purified DNA can be used for any downstream molecular biology applications.
Features and Benefits
|
DNA purity |
OD 260/280≥1.8 |
|
DNA yield |
Up to 1.2 mg/200 ml LB Broth |
|
Elution volume |
1-3 ml |
|
Flexible |
Vacuum or spin format |
Performance
Kit Components
|
Components |
M1010-10 |
M1010-30 |
|
Resuspension Buffer |
125 ml |
375 ml |
|
Lysis Buffer |
125 ml |
375 ml |
|
Neutralization Buffer |
125 ml |
375 ml |
|
DNA Binding Buffer |
95 ml |
285 ml |
|
Column Prep. Buffer |
125 ml |
375 ml |
|
Wash Buffer I |
125 ml |
375 ml |
|
Wash Buffer II |
25 ml |
75 ml |
|
Elution Buffer |
40 ml |
120 ml |
|
RNase A |
1 tube |
3 tubes |
|
Spin Column Units |
10 |
30 |
|
EZ Filter Syringe |
10 |
30 | |
