High MW Tissue DNA Isolation

EZ High MW Tissue DNA Isolation Kit

EZ High MW Tissue DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from solid tissues. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The tissues are digested with Proteinase K in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The kit can process a total of 4 gram solid tissue. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).

Features and Benefits

DNA Purity:
DNA Size Range:
DNA Yield:
Convenient:
Safe Handling:

Typical O.D 260/280 >1.8, free of RNA
>50 kb, typical ~100-200 kb
0.5-10 µg/mg tissue
Scalable protocols
No Phenol/Chloroform extraction

Performance

High Molecular Weight DNA Purified from Tissues Using EZ High MW Tissue DNA Isolation Kit

M Liver Heart Lung Kidney

Genomic DNA isolated from different tissues using EZ High MW Tissue DNA Isolation Kit were analyzed on 1% agarose gel. M: Lambda-HindIII Ladder.

Kit Components

Components	M1008-1	M1008-4
Nuclei Lysis Solution	32 ml	125 ml
Protein Precipitation Solution	13 ml	50 ml
DNA Hydration Solution	25 ml	100 ml
Proteinase K Solution	1 ml	4 ml
RNaseA Solution	0.17 ml	0.65 ml