High MW Tail DNA Isolation

 

EZ High MW Mouse Tail DNA Isolation Kit

 

EZ High MW Mouse Tail DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from mouse tails. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The mouse tail is digested with Proteinase K in Nuclei Lysis Solution. Contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).

  

Features and Benefits

 

DNA Purity: DNA Size Range: DNA Yield: Safe Handling:

Typical O.D 260/280 >1.8 >50 kb, typical ~100-200 kb 0.5-5 µg/mg tail tissue No Phenol/Chloroform extraction

   

Performance

 

High Molecular Weight DNA Purified from Mouse Tail Using EZ High MW Mouse Tail DNA Isolation Kit

      M         1         2          3

 

 

 

 

 

 

 

Genomic DNA (lanes 1-3) was isolated from mouse tail using EZ High MW Mouse Tail DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII ladder.

  

Kit Components

 

Components	M1007-100	M1007-400
Nuclei Lysis Solution	32 ml	125 ml
Protein Precipitation Solution	13 ml	50 ml
DNA Hydration Solution	25 ml	100 ml
Proteinase K Solution	1 ml	4 ml