High MW Cell DNA Isolation

EZ High MW Cell DNA Isolation Kit

EZ High MW Cell DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from cultured cells. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The cells are lysed in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The kit can process a total number of 8 x 10^8 cells. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).

Features and Benefits

DNA Purity:
DNA Size Range:
DNA Yield:
Convenient:
Safe Handling:

Typical O.D 260/280 >1.8
>50 kb, typical ~100-200 kb
5-10 µg/1-2 x 10^6 cells
Scalable protocols
No Phenol/Chloroform extraction

Performance

High Molecular Weight DNA Purified from cells Using EZ High MW Cell DNA Isolation Kit

M 1 2

Genomic DNA (lanes 1-2) was isolated from NIH 3T3 Cells using EZ High MW Cell DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII Ladder.

Kit Components

Components	M1006-100	M1006-400
Nuclei Lysis Solution	32 ml	125 ml
Protein Precipitation Solution	13 ml	50 ml
DNA Hydration Solution	25 ml	100 ml
RNaseA Solution	0.17 ml	0.65 ml