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EZ High MW Blood DNA Isolation Kit


EZ High MW Blood DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic, mitochondrial or viral DNA from fresh or frozen whole blood and buffy coat. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The red blood cells which lack DNA are first lysed in RBC Lysis Solution and separated from white cells by centrifugation. The white cells are then lysed in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).  
Features and Benefits

DNA Purity:

Typical O.D 260/280 1.8

DNA Size Range:

>50 kb, typical ~100-200 kb

DNA Yield:

16-50 g/7x10^6 white cells


Scalable protocols

Safe Handling:

No Phenol/Chloroform extraction

High Molecular Weight DNA Purified from Blood Using EZ High MW Blood DNA Isolation Kit
    M           1          2         3         4
Genomic DNA (lanes 1-4) was purified from sheep blood using EZ High MW Blood DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII ladder.
Kit Components

Kit Components




RBC Lysis Solution

100 ml

3000 ml

3000 ml

Nuclei Lysis Solution

35 ml

1000 ml

1000 ml

Protein Precipitation Solution

12 ml

350 ml

350 ml

DNA Hydration Solution

10 ml

110 ml

110 ml

RNaseA Solution

0.25 ml


5 ml

Products (Total Items: 3)

M1005-30 High MW Blood DNA Isolation Kit 30 ml Blood $80.00
M1005-1000 High MW Blood DNA Isolation Kit 1000 ml Blood $798.00
M1005-1001 High MW Blood DNA Isolation Kit Plus RNase A 1000 ml Blood $898.00

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