High MW Blood DNA Isolation

EZ High MW Blood DNA Isolation Kit

EZ High MW Blood DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic, mitochondrial or viral DNA from fresh or frozen whole blood and buffy coat. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The red blood cells which lack DNA are first lysed in RBC Lysis Solution and separated from white cells by centrifugation. The white cells are then lysed in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).

Features and Benefits

DNA Purity:	Typical O.D 260/280 =1.8
DNA Size Range:	>50 kb, typical ~100-200 kb
DNA Yield:	16-50 µg/7x10^6 white cells
Convenient:	Scalable protocols
Safe Handling:	No Phenol/Chloroform extraction

Performance

High Molecular Weight DNA Purified from Blood Using EZ High MW Blood DNA Isolation Kit

M 1 2 3 4

Genomic DNA (lanes 1-4) was purified from sheep blood using EZ High MW Blood DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII ladder.

Kit Components

Kit Components	M1005-30	M1005-1000	M1005-1001
RBC Lysis Solution	100 ml	3000 ml	3000 ml
Nuclei Lysis Solution	35 ml	1000 ml	1000 ml
Protein Precipitation Solution	12 ml	350 ml	350 ml
DNA Hydration Solution	10 ml	110 ml	110 ml
RNaseA Solution	0.25 ml	-	5 ml