EZ High MW Blood DNA Isolation Kit
EZ High MW Blood DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic, mitochondrial or viral DNA from fresh or frozen whole blood and buffy coat. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The red blood cells which lack DNA are first lysed in RBC Lysis Solution and separated from white cells by centrifugation. The white cells are then lysed in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).
Features and Benefits
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DNA Purity: |
Typical O.D 260/280 ≥1.8 |
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DNA Size Range: |
>50 kb, typical ~100-200 kb |
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DNA Yield: |
16-50 µg/7x10^6 white cells |
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Convenient: |
Scalable protocols |
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Safe Handling: |
No Phenol/Chloroform extraction |
Performance
High Molecular Weight DNA Purified from Blood Using EZ High MW Blood DNA Isolation Kit
M 1 2 3 4
Genomic DNA (lanes 1-4) was purified from sheep blood using EZ High MW Blood DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII ladder.
Kit Components
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Kit Components |
M1005-30 |
M1005-1000 |
M1005-1001 |
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RBC Lysis Solution |
100 ml |
3000 ml |
3000 ml |
|
Nuclei Lysis Solution |
35 ml |
1000 ml |
1000 ml |
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Protein Precipitation Solution |
12 ml |
350 ml |
350 ml |
|
DNA Hydration Solution |
10 ml |
110 ml |
110 ml |
|
RNaseA Solution |
0.25 ml |
- |
5 ml |
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