High MW Bacterial DNA Isolation

EZ High MW Bacterial DNA Isolation Kit

The EZ High MW Bacterial DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from Gram Positive and Gram Negative Bacteria. The kit utilizes a solution based salting-out precipitation method for purification of DNA. Bacterial wall is lysed by Lytic Enzyme Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).

Features and Benefits

DNA Purity:	O.D 260/280 =1.8
DNA Size Range:	>50 kb, typical ~100-200 kb
DNA Yield:	10-20 µg/ml overnight cluture
Safe Handling:	No Phenol/Chloroform extraction

Performance

High Molecular Weight DNA purified from Bacteria Using EZ High MW Bacterial DNA Isolation Kit

1 2 3 M

Genomic DNA (Lanes 1-3) was isolated from Ecoli DH5a using EZ High MW Bacterial DNA Isolation Kit and analyzed on a 0.8% Agarose gel. M: 1Kb Ladder.

Kit Components

Components	M1012-50	M1012-100
Cell Suspension Solution	30 ml	60 ml
Nuclei Lysis Solution	30 ml	60 ml
Protein Precipitation Solution	10 ml	20 ml
DNA Hydration Solution	10 ml	20 ml
RNaseA Solution	0.15 ml	0.3 ml
Lysozyme	1 tube	2 tubes
Lysozyme Re-suspension Solution	3 ml	3 ml x 2