(0)  item(s) in your cart Total: $0
Tel: 1(800) 637-0262  Fax: 1(877) 693-4868
Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
Science Kits
EZ High MW Bacterial DNA Isolation Kit


The EZ High MW Bacterial DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from Gram Positive and Gram Negative Bacteria. The kit utilizes a solution based salting-out precipitation method for purification of DNA. Bacterial wall is lysed by Lytic Enzyme Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).  
Features and Benefits

DNA Purity:

O.D 260/280 1.8

DNA Size Range:

>50 kb, typical ~100-200 kb

DNA Yield:

10-20 g/ml overnight cluture

Safe Handling:

No Phenol/Chloroform extraction

High Molecular Weight DNA purified from Bacteria Using EZ High MW Bacterial DNA Isolation Kit
      1           2           3         M
Genomic DNA (Lanes 1-3) was isolated from Ecoli DH5α using EZ High MW Bacterial DNA Isolation Kit and analyzed on a 0.8% Agarose gel. M: 1Kb Ladder.
Kit Components
Cell Suspension Solution
30 ml
60 ml
Nuclei Lysis Solution
30 ml
60 ml
Protein Precipitation Solution
10 ml
20 ml
DNA Hydration Solution
10 ml
20 ml
RNaseA Solution
0.15 ml
0.3 ml
1 tube
2 tubes
Lysozyme Re-suspension Solution
3 ml
3 ml x 2
Products (Total Items: 2)

M1012-50 High MW Bacterial DNA Isolation Kit 50 preps $56.00
M1012-100 High MW Bacterial DNA Isolation Kit 100 preps $106.00

Copyright 2006 EZ BioResearch LLC LLC. All Rights Reserved.