Gel Extraction

EZ Gel Extraction Kit

EZ Gel Extraction Kit is a silica membrane based spin column format kit for isolating DNA fragments from all types of agarose gels and buffer systems. This kit can also be used for purifying DNA from enzymatic reactions. The purified DNA can be used for any downstream molecular biology applications.

Features and Benefits

DNA Size Range:
Spin Column Binding Capacity:
Types of Agaroses:
Types of Gel Buffers:
Elution Volume:
Recovery Rate:

Purify DNA fragments 100 bp-10 kb in size
Up to 50 µg DNA
All
All
50 µl
75-98%

Performance

High recovery of DNA fragments from gels with EZ Gel Extraction Kit

A B

DNA fragments (size indicated) before and after extraction with EZ Gel Extraction Kit. A. Lane 1, 1 Kb MW marker; Lane 2, DNA fragments before extraction. The amount of DNA loaded equals 90% recovery rate after extraction. Lane 3-5, DNA fragments after gel extraction. Samples were analyzed on a 2% agarose gel with TAE buffer. B. Lane 1, 1 Kb MW marker; Lane 2, DNA fragments before extraction. The amount of DNA loaded equals 85% recovery rate after extraction. Lane 3-5, DNA fragments after gel extraction. Samples were analyzed on a 0.8% agarose gel with TAE buffer.

Kit Components

Components	M1002-50	M1002-250
DNA Binding Buffer	48 ml	115 ml x 2
Wash Buffer	6 ml	15 ml x 2
Elution Buffer	8 ml	20 ml
Spin Column Units	50	250
Sample Collection Tubes	50	250