Eco-Direct Tissue/Tail Genotyping

EZ Eco-Direct Tissue/Tail PCR Genotyping Kit

EZ Eco-Direct Tissue/Tail PCR Genotyping Kit is designed for rapid extraction and amplification of genomic DNA from mouse tail/toe/ear punch and animal tissues. With the EZ Eco-Direct Tissue/Tail PCR Genotyping Kit the extraction of genomic DNA is done by heating the sample in Extraction Solution I at 95^oC for 15 min and then mixed with Extraction Solution II prior to PCR. An aliquot of the DNA extraction is mixed with a 2X PCR Ready Mix III included in the kit and user-selected primers to amplify target DNA. The 2X PCR Ready Mix III containing buffer, dNTP and Taq polymerase is specially optimized for use with the extracted DNA. The 2X PCR Ready Mix III contains an inert red dye that allows direct loading of PCR products onto agarose gels to visualize amplified target.

Features and Benefits

Fast Protocol:

Multiplexing:

Significant savings:

Consistent results:

Genomic DNA ready for PCR in 15 min. No overnight digestion

Strip tube for parallel processing

Cost-effective, no need to purify genomic DNA with expensive spin columns.

Virtually 100% success rate!

Performance

PCR analysis of DNA extracted from mouse tails using EZ Eco-Direct Tissue/Tail PCR Genotyping Kit

Mouse genomic DNA was extracted from eight mouse tails and PCR amplification of the two target genes was carried out using EZ Eco-Direct Tissue/Tail PCR Genotyping Kit. The amplified DNA fragments (220 bp in lanes 1-8 and 498 bp in lanes 1”-8”) were analyzed on a 2% agarose gel. M: 100 bp DNA Even Ladder.

Kit Components

Components	ECO-100	ECO-500	ECO-1000
Extraction Solution I	6 ml	30 ml	2 x 30 ml
Extraction Solution II	6 ml	30 ml	2 x 30 ml
2X PCR Ready Mix III	1 ml	5 x 1 ml	10 x 1 ml