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EZ High MW Cell DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from cultured cells. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The cells are lysed in Nulcei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The kit can process a total number of 8 x 10^8 cells. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots). |
