EZ Blood Total RNA Isolation Kit
EZ Blood Total RNA Isolation Kit is designed for isolation of high quality total RNA from up to 1.5 ml fresh whole blood. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. Erythrocytes are first lysed in the RBC Lysis Solution. The leukocytes are recovered and lysed in the RNA Lysis/Binding Buffer. After pre-cleaned up, the lysate is applied to the Spin Column. The contaminated genomic DNA is removed by DNase digestion. Any other impurities are removed by stringent washing steps. The purified RNA is eluted in RNase-Free Water. The kit eliminates the need for toxic organic extraction and time-consuming ethanol precipitation steps required in the other RNA purification methods. The purified RNA is virtually free of Genomic DNA contamination and is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
Features and Benefits
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RNA Purity: |
O.D260/280≥1.9 |
|
RNA Yield: |
~3 µg/ml fresh blood |
|
RNA Size: |
>200 nucleotides |
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Spin Column Binding Capacity: |
Up to 200 µg RNA |
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Maximal Starting Material: |
Up to 1.5 ml fresh blood |
|
Elution Volume: |
50 µl |
Performance
High quality RNA (virtually no genomic DNA) isolated from blood with EZ Blood Total RNA Isolation Kit
M Q EZ 
-Genomic DNA
Total RNA was isolated from 1 ml fresh blood and analyzed on 0.8% agarose gel. Lane Q: RNA isolated with competitor Q RNA Blood Mini Kit. Lane EZ: RNA isolated with EZ Blood Total RNA Isolation Kit. M: RNA ladder.
Kit Components
|
Components |
R1005-50 |
|
RBC Lysis Solution |
450 ml |
|
RNA Lysis/Binding Buffer |
30 ml |
|
Wash Buffer I |
47.5 ml |
|
Wash Buffer II |
10 ml |
|
RNase-Free DNase I |
1 tube |
|
DNase Reconstitution Buffer |
0.5 ml |
|
10 X DNase Digestion Buffer |
0.6 ml |
|
RNase-Free Water |
10 ml |
|
Pre-Clean Column Units |
50 |
|
Spin Column Units |
50 |
|
Collection Tubes (2 ml) |
50 |
|
Collection Tubes (1.5 ml) |
50 |
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