(0)  item(s) in your cart Total: $0
Tel: 1(800) 637-0262  Fax: 1(877) 693-4868
Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
Science Kits
EZ Blood Total RNA Isolation Kit 
EZ Blood Total RNA Isolation Kit is designed for isolation of high quality total RNA from up to 1.5 ml fresh whole blood. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. Erythrocytes are first lysed in the RBC Lysis Solution. The leukocytes are recovered and lysed in the RNA Lysis/Binding Buffer. After pre-cleaned up, the lysate is applied to the Spin Column. The contaminated genomic DNA is removed by DNase digestion. Any other impurities are removed by stringent washing steps. The purified RNA is eluted in RNase-Free Water. The kit eliminates the need for toxic organic extraction and time-consuming ethanol precipitation steps required in the other RNA purification methods. The purified RNA is virtually free of Genomic DNA contamination and is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.   
Features and Benefits

RNA Purity:


RNA Yield:

~3 g/ml fresh blood

RNA Size:

>200 nucleotides

Spin Column Binding Capacity:

Up to 200 g RNA

Maximal Starting Material:

Up to 1.5 ml fresh blood

Elution Volume:

50 l

High quality RNA (virtually no genomic DNA) isolated from blood with EZ Blood Total RNA Isolation Kit
       M         Q          EZ
-Genomic DNA
Total RNA was isolated from 1 ml fresh blood and analyzed on 0.8% agarose gel. Lane Q: RNA isolated with competitor Q RNA Blood Mini Kit. Lane EZ: RNA isolated with EZ Blood Total RNA Isolation Kit. M: RNA ladder.
Kit Components
RBC Lysis Solution
450 ml
RNA Lysis/Binding Buffer
30 ml
Wash Buffer I
47.5 ml
Wash Buffer II
10 ml
RNase-Free DNase I
1 tube
DNase Reconstitution Buffer 
0.5 ml
10 X DNase Digestion Buffer
0.6 ml
RNase-Free Water
10 ml
Pre-Clean Column Units
Spin Column Units
Collection Tubes (2 ml)
Collection Tubes (1.5 ml)


Products (Total Items: 1)

R1005-50 EZ Blood Total RNA Isolation Kit 50 preps $249.00

Copyright 2006 EZ BioResearch LLC LLC. All Rights Reserved.