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Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates for Bacterial Growth
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
 
EZ Tissue/Cell Total RNA Miniprep Kit
 
EZ Tissue/Cell Total RNA Mini Kit is designed for purification of high quality total RNA from variety of mammalian tissues or cultured cells. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The contaminated genomic DNA is removed by DNase digestion. Any other impurities are removed by stringent washing steps. The purified RNA is eluted in RNase-Free Water. The kit eliminates the need for toxic organic extraction and time-consuming ethanol precipitation steps required in the other RNA purification methods. The purified RNA is virtually free of Genomic DNA contamination and is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
  
Features and Benefits
 

RNA Purity:

O.D260/2801.9

RNA Yield:

2-4 g/mg tissue or 10-25 g/1 x 10^6 cells

RNA Size:

>200 nucleotides

Spin Column Binding Capacity:

Up to 200 g RNA

Maximal Starting Material:

Up to 30 mg of tissue or 1 x 10^7 cells

Elution Volume:

50 l

 
Performance

            Agarose gel electrophresis analysis of RNA isolated from different sources using EZ Tissue/Cell RNA Miniprep Kit
 
 
            Real time RT-PCR analysis of various amounts of RNA isolated from 3 different preps using EZ Tissue/Cell RNA Miniprep Kit.
 
 
Kit Components
 

Components

R1002-50

R1002-250

RNA Lysis/Binding Buffer

30 ml

75 ml x 2

Wash Buffer I

47.5 ml

118.75 ml x 2

Wash Buffer II

10 ml

25 ml x 2

RNase-Free Water

10 ml

20 ml

Pre-Clean Column Units

50

250

Spin Column Units

50

250

Collection Tubes (2 ml)

50

250

Collection Tubes (1.5 ml)

50

250

  
Products (Total Items: 4)

R1002-50 EZ Tissue/Cell Total RNA Mini Kit 50 preps $169.00
R1002-250 EZ Tissue/Cell Total RNA Mini Kit 250 preps $744.00
R1002-50-pre Pre-Clean Spin Columns (50) for RNA Kits 50 $30.00
R1002-250-Pre Pre-Clean Spin Columns (250) for RNA Kits 250 $145.00

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