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Tissue/Tail PCR Genotyping
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Tissue/Tail DNA Isolation
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EZ Tissue/Tail DNA Isolation Plus PCR Kit
 
EZ Tissue/Tail DNA Isolation Plus PCR Kit is designed for isolating genomic DNA from animal tissues including rodent tails and PCR genotyping of purified genomic DNA. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with Proteinase K in a specially formulated lysis buffer that allows protein denaturation. Residual RNA is removed by RNase digestion. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting. The 2X PCR Ready Mix included in the kit is specially formulated for genotyping. The enhanced formulation leads to less false negatives in genotyping. The 2X PCR Ready Mix contains an inert red dye that allows direct loading of PCR products onto agarose gels and eliminates the need for addition of loading dye.

 

Features and Benefits
 
DNA Purity:
Typical O.D 260/280 1.8, free of RNA
DNA Size Range:
>20 kb
DNA Yield:
5-30 g
Spin Column Binding Capacity:
50 g DNA
Elution Volume:
200 l
   
Performance
 
High Quality DNA purified from different tissues with EZ Tissue/Tail DNA Isolation Kit
 
     M     1     2     3     4     5     6     7     8   

   

Genomic DNA isolated from mouse tissues using EZ Tissue/Tail DNA Isolation Kit was digested with (Lanes 2,4,6,8) or without (Lanes 1,3,5,7) EcoRI and analyzed on a 0.8% agarose gel. Lanes 1,2: tail; Lanes 3,4: liver; Lanes 5,6: kidney; Lanes7,8: spleen. M: Lambda-HindIII ladder.
 
Typical DNA Yields Using EZ Tissue/Tail DNA Isolation Kit

Tissue Source

Starting Amount

DNA Yield (ug)

Mouse Tail

0.5-1.2 cm

5-15

Liver

25 mg

15-30

Kidney

25 mg

10-25

Heart

25 mg

5-10

Lung

25 mg

5-10

Spleen

10 mg

5-25

Brain

25 mg

15-30

 
Kit Components
 

Components

M1003-100

Tissue Lysis Buffer

22 ml

DNA Binding Buffer

25 ml

Wash Buffer I

30 ml

Wash Buffer II

18 ml

Elution Buffer

50 ml

Proteinase K

2 ml

RNaseA

2 ml

Spin Column Units

100

Sample Collection Tubes

100

2X PCR Ready Mix

1 ml

Products (Total Items: 4)

G1003-100 Tissue/Tail DNA Isolation Plus PCR Kit 100 preps $196.00
P1000-100 2X PCR Ready Mix For Genotyping 100 reactions $56.00
P1000-300 2X PCR Ready Mix For Genotyping 300 reactions, 50% discount shipping $163.00
P1000-1000 2X PCR Ready Mix For Genotyping 1000 reactions, Free Shipping $532.00

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