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Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
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Tissue/Tail DNA + PCR Kit
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Tissue/Tail DNA Isolation
-EZ Tissue DNA Isolation Kit Components
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
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EZ Tissue/Tail DNA Isolation Kit
 
EZ Tissue/Tail DNA Isolation Kit is designed for isolating genomic DNA from animal tissues including rodent tails. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with Proteinase K in a specially formulated lysis buffer that allows protein denaturation. Residual RNA is removed by RNase digestion. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting.

 

Features and Benefits 

DNA Purity:
DNA Size Range:
DNA Yield:
Spin Column Binding Capacity:
Elution Volume:
Typical O.D 260/280 > 1.8, free of RNA
> 20 kb
5-30 g
50 g
200 l

Performance
 
High Quality DNA purified from different tissues with EZ Tissue/Tail DNA Isolation Kit
     M     1     2     3     4     5     6     7     8   

   

Genomic DNA isolated from mouse tissues using EZ Tissue/Tail DNA Isolation Kit was digested with (Lanes 2,4,6,8) or without (Lanes 1,3,5,7) EcoRI and analyzed on a 0.8% agarose gel. Lanes 1,2: tail; Lanes 3,4: liver; Lanes 5,6: kidney; Lanes7,8: spleen. M: Lambda-HindIII ladder.
 
Typical DNA Yields Using EZ Tissue/Tail DNA Isolation Kit
 

Tissue Source

Starting Amount

DNA Yield (ug)

Mouse Tail

0.5-1.2 cm

5-15

Liver

25 mg

15-30

Kidney

25 mg

10-25

Heart

25 mg

5-10

Lung

25 mg

5-10

Spleen

10 mg

5-25

Brain

25 mg

15-30

  
Kit Components
 

Components

M1003-50

M1003-250

Tissue Lysis Buffer

11 ml

50 ml

DNA Binding Buffer

13 ml

55 ml

Wash Buffer I

15 ml

62.5 ml

Wash Buffer II

9 ml

37.5 ml

Elution Buffer

25 ml

110 ml

Proteinase K

1 ml

5 ml

RNaseA

1 ml

5 ml

Spin Column Units

50

250

Sample Collection Tubes

50

250

 

Sub-Categories
EZ Tissue DNA Isolation Kit Components

Products (Total Items: 2)

M1003-50 EZ Tissue/Tail DNA Isolation Kit 50 preps $87.00
M1003-250 EZ Tissue/Tail DNA Isolation Kit 250 preps $360.00

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