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Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates for Bacterial Growth
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
 
EZ SV Tissue/Tail PCR Genotyping Kit
(Small Volume Extraction Format)
 
EZ SV Tissue/Tail Genotyping PCR Kit is the second generation of EZ Fast Tissue/Tail PCR Genotyping Kit which is designed for rapid small volume extraction and amplification of genomic DNA from mouse tail/toe/ear punch and animal tissues.  With automated one-solution extraction, extraction of genomic DNA from animal tissue is as easy as pushing a start button. No termination buffer is needed. There is no need for overnight digestion, Phenol/Chloroform extraction and laborious DNA purification. Neither there is requirement for use of expensive DNA spin columns. Most importantly of all, the consistent result from EZ SV Genotyping Kit is unmatched by any other DNA extraction-based genotyping methods available in the market! The kit includes Tissue Lysis Solution A and B and 2X PCR Ready Master Mix III. The 2x PCR Ready Master Mix III containing Taq polymerase and dNTPs is specially optimized for use with the extracted DNA. It also contains an inert red dye that allows direct loading of PCR products onto agarose gels without addition of loading dye.
 
Extraction Protocol:
1) Add DNA Extraction Buffer to the Strip tube with a tail sample.
2) Place the strip tube in a thermocyler and start the extraction program
3) After 20-min, take out the strip tube and invert 5 times.
Extraction is done and genomic DNA is ready for PCR.
Features and Benefits 
Automated extraction:
Fast Protocol:
Multiplexing:
Easy Handling:
Significant savings:
Consistent results:
Push-button DNA extraction with <10-second hands-on time.
Genomic DNA ready for PCR in 20 min. No overnight digestion
Strip tube for parallel processing
One solution extraction. No termination buffer
No need to purify genomic DNA with expensive spin columns.
Virtually 100% success rate!
Performance
Consistency is one of the important factors in choosing a genotyping method. Due to unoptimized genotyping conditions for many commercial and home-made methods or protocols, the PCR analysis sometimes give weak and/or ambiguous bands. Either, one has to repeat the experiment. Or worse, one misreads the data and carries out downstream experiments. In either case, days or months of time and efforts are wasted. Like the EZ Fast Tissue/Tail PCR Genotyping Kit, EZ SV Tissue/Tail PCR genotyping gives much stronger signals from PCR reactions and offers no ambiguous results. Consistency in results will save time and effort in a long run.
 
 
 
 
 
 
 
 
 
 
 
 
PCR analysis of DNA extracted from mouse tail samples using EZ SV Tissue/Tail PCR Genotyping Kit
DNA was extracted from six mouse tails and PCR amplification of the three target genes was carried out using the EZ SV Tissue/Tail PCR Genotyping Kit. The three amplified DNA fragments (220 bp in lanes 1-6, 498 bp in lanes 1'-6' and 983 bp in lanes 1"-6") were analyzed on a 2% agarose gel. M: 100 bp DNA Even Ladder.
 
Kit Components
 
Components
GSV-100
GSV-500
GSV-1000
Tissue Lysis Solution A
1 ml
5 x 1 ml
10 x 1 ml
Tissue Lysis Solution B
10 ml
5 x 10 ml
10 x 10 ml
2X PCR Ready Mix III
1 ml
5 x 1 ml
10 x 1 ml
Products (Total Items: 7)

GSV-10 EZ SVTissue/Tail PCR Genotyping Kit (Trial Kit) 10 reactions $19.95
GSV-100 EZ SV Tissue/Tail PCR Genotyping Kit 100 reactions $67.00
GSV-500 EZ SV Tissue/Tail PCR Genotyping Kit 500 reactions $327.00
GSV-1000 EZ SV Tissue/Tail PCR Genotyping Kit 1000 reactions, Free Shipping $624.00
P4000-100 2X PCR Ready Mix III for Genotyping 100 reactions $46.00
P4000-500 2X PCR Ready Mix III for Genotyping 500 reactions $163.00
P4000-1000 2X PCR Ready Mix III for Genotyping 1000 reactions $305.00

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