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EZ RNA Clean-Up Kit
 
EZ RNA Clean Up Kit can be used to clean up RNA from crude RNA preps or after enzymatic reactions such as labeling reaction, RNA ligation or DNase digestion. The kit is designed based on selective binding of RNA to silica membrane in the presence of chaotropic salts. The crude RNA is mixed with RNA Lysis/Binding Buffer and the mixture is then loaded onto the silica membrane-based spin column. Any impurities are removed by subsequent washing steps. The purified RNA is eluted in RNase-Free Water. The purified RNA is suitable for conventional RT-PCR, real time quantitative RT-PCR, northern blotting, RNase protection assay, RNA amplification for microarray analysis.
 
Features and Benefits

RNA Purity:

O.D260/2801.9

RNA Size:

>200 nucleotides

Spin Column Binding Capacity:

Up to 200 µg RNA

Elution Volume:

50 µl

 
Performance
 
 
High Quality RNA with E         Total RNA was isolated from mouse liver using phenol–based RNA extraction method and was then purified with EZ RNA Clean-Up Kit. The RNA was analyzed with Agilent 2100 Bioanalyzer.
 
Kit Components

Components

R1001-50

R1001-250

RNA Lysis/Binding Buffer

20 ml

90 ml

Wash Buffer

8 ml x 2

25 ml x 3

RNase-Free Water

10 ml

20 ml

Spin Column Units

50

250

Collection Tubes (2 ml)

50

250

Collection Tubes (1.5 ml)

50

250

 
 
 
 
 
Products (Total Items: 2)

R1001-50 EZ RNA Clean Up Kit 50 preps $148.00
R1001-250 EZ RNA Clean Up Kit 250 preps $729.00

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