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Tissue/Tail PCR Genotyping
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EZ Plasmid Maxiprep Kit
 
EZ Plasmid Maxiprep Kit provides a simple and fast silica membrane based method to isolate high quality plasmid DNA from large recombinant E. coli culture. The principle of the kit is based on alkaline/SDS lysis of bacteria cells followed by selective adsorption of plasmid DNA on silica membrane in the presence of high salt. Contaminants are removed by wash buffers and the plasmid DNA is eluted in Elution Buffer. Up to 1.2 mg of plasmid DNA can be isolated from 200 ml overnight culture grown in LB broth. The isolated plasmid DNA is predominantly in its super-coiled form. The purified DNA can be used for any downstream molecular biology applications.
 
Features and Benefits
DNA purity
OD260/280≥1.8
DNA yield
Up to 1.2 mg/200 ml LB Broth
Elution volume
3 ml
Flexible
Vacuum or spin format

Performance

 

 

Kit Components
 

Components

M1020-10

M1020-30

Resuspension Buffer

125 ml

375 ml

Lysis Buffer

125 ml

375 ml

Neutralization Buffer

125 ml

375 ml

DNA Binding Buffer

95 ml

285 ml

Column Prep. Buffer

125 ml

375 ml

Wash Buffer I

125 ml

375 ml

Wash Buffer II

25 ml

75 ml

Elution Buffer

40 ml

120 ml

RNase A

1 tube

3 tubes

Spin Column Units

10

30

Products (Total Items: 3)

M1020-10 EZ Plasmid Maxiprep Kit 10 preps $119.00
M1020-30 EZ Plasmid Maxiprep Kit 30 preps $321.00
M1010-COL EZ Maxiprep Spin Column 1 column $10.50

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