EZ High MW Mouse Tail DNA Isolation Kit
EZ High MW Mouse Tail DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from mouse tails. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The mouse tail is digested with Proteinase K in Nuclei Lysis Solution. Contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).
Features and Benefits
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DNA Purity: |
Typical O.D 260/280 ≥1.8 |
|
DNA Size Range: |
>50 kb, typical ~100-200 kb |
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DNA Yield: |
0.5-5 µg/mg tail tissue |
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Safe Handling: |
No Phenol/Chloroform extraction |
Performance
High Molecular Weight DNA Purified from Mouse Tail Using EZ High MW Mouse Tail DNA Isolation Kit
M 1 2 3
Genomic DNA (lanes 1-3) was isolated from mouse tail using EZ High MW Mouse Tail DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII ladder.
Kit Components
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Components |
M1007-100 |
M1007-400 |
|
Nuclei Lysis Solution |
32 ml |
125 ml |
|
Protein Precipitation Solution |
13 ml |
50 ml |
|
DNA Hydration Solution |
25 ml |
100 ml |
|
Proteinase K Solution |
1 ml |
4 ml | |
