EZ High MW Cell DNA Isolation Kit
EZ High MW Cell DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from cultured cells. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The cells are lysed in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The kit can process a total number of 8 x 10^8 cells. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).
Features and Benefits
|
DNA Purity: |
Typical O.D 260/280 ≥1.8 |
|
DNA Size Range: |
>50 kb, typical ~100-200 kb |
|
DNA Yield: |
5-10 µg/1-2 x 10^6 cells |
|
Convenient: |
Scalable protocols |
|
Safe Handling: |
No Phenol/Chloroform extraction |
Performance
High Molecular Weight DNA Purified from cells Using EZ High MW Cell DNA Isolation Kit
M 1 2
Genomic DNA (lanes 1-2) was isolated from NIH 3T3 Cells using EZ High MW Cell DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII Ladder.
Kit Components
|
Components |
M1006-100 |
M1006-400 |
|
Nuclei Lysis Solution |
32 ml |
125 ml |
|
Protein Precipitation Solution |
13 ml |
50 ml |
|
DNA Hydration Solution |
25 ml |
100 ml |
|
RNaseA Solution |
0.17 ml |
0.65 ml | |
