(0)  item(s) in your cart Total: $0
Tel: 1(800) 637-0262  Fax: 1(877) 693-4868
Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates for Bacterial Growth
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
 
EZ High MW Blood DNA Isolation Kit

  

EZ High MW Blood DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic, mitochondrial or viral DNA from fresh or frozen whole blood and buffy coat. The kit utilizes a solution based salting-out precipitation method for purification of DNA. The red blood cells which lack DNA are first lysed in RBC Lysis Solution and separated from white cells by centrifugation. The white cells are then lysed in Nuclei Lysis Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).  
 
Features and Benefits
 

DNA Purity:

Typical O.D 260/280 1.8

DNA Size Range:

>50 kb, typical ~100-200 kb

DNA Yield:

16-50 g/7x10^6 white cells

Convenient:

Scalable protocols

Safe Handling:

No Phenol/Chloroform extraction

  
Performance
 
High Molecular Weight DNA Purified from Blood Using EZ High MW Blood DNA Isolation Kit
    M           1          2         3         4
 
 
 
 
 
 
 
Genomic DNA (lanes 1-4) was purified from sheep blood using EZ High MW Blood DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII ladder.
 
Kit Components
 

Kit Components

M1005-30

M1005-1000

M1005-1001

RBC Lysis Solution

100 ml

3000 ml

3000 ml

Nuclei Lysis Solution

35 ml

1000 ml

1000 ml

Protein Precipitation Solution

12 ml

350 ml

350 ml

DNA Hydration Solution

10 ml

110 ml

110 ml

RNaseA Solution

0.25 ml

-

5 ml

Products (Total Items: 3)

M1005-30 High MW Blood DNA Isolation Kit 30 ml Blood $80.00
M1005-1000 High MW Blood DNA Isolation Kit 1000 ml Blood $798.00
M1005-1001 High MW Blood DNA Isolation Kit Plus RNase A 1000 ml Blood $898.00

Copyright 2006 EZ BioResearch LLC LLC. All Rights Reserved.