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EZ High MW Bacterial DNA Isolation Kit

  

The EZ High MW Bacterial DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from Gram Positive and Gram Negative Bacteria. The kit utilizes a solution based salting-out precipitation method for purification of DNA. Bacterial wall is lysed by Lytic Enzyme Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).  
 
Features and Benefits
 

DNA Purity:

O.D 260/280 1.8

DNA Size Range:

>50 kb, typical ~100-200 kb

DNA Yield:

10-20 g/ml overnight cluture

Safe Handling:

No Phenol/Chloroform extraction

  
Performance
 
High Molecular Weight DNA purified from Bacteria Using EZ High MW Bacterial DNA Isolation Kit
 
      1           2           3         M
Genomic DNA (Lanes 1-3) was isolated from Ecoli DH5α using EZ High MW Bacterial DNA Isolation Kit and analyzed on a 0.8% Agarose gel. M: 1Kb Ladder.
 
Kit Components
 
 Components
M1012-50
M1012-100
Cell Suspension Solution
30 ml
60 ml
Nuclei Lysis Solution
30 ml
60 ml
Protein Precipitation Solution
10 ml
20 ml
DNA Hydration Solution
10 ml
20 ml
RNaseA Solution
0.15 ml
0.3 ml
Lysozyme
1 tube
2 tubes
Lysozyme Re-suspension Solution
3 ml
3 ml x 2
Products (Total Items: 2)

M1012-50 High MW Bacterial DNA Isolation Kit 50 preps $56.00
M1012-100 High MW Bacterial DNA Isolation Kit 100 preps $106.00

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