EZ High MW Bacterial DNA Isolation Kit
The EZ High MW Bacterial DNA Isolation Kit is for isolating high molecular weight, archive-quality genomic DNA from Gram Positive and Gram Negative Bacteria. The kit utilizes a solution based salting-out precipitation method for purification of DNA. Bacterial wall is lysed by Lytic Enzyme Solution. RNA is removed by treatment of RNaseA. Other contaminants such as proteins are removed by salt precipitation. The DNA is recovered by precipitation with alcohol and dissolved in DNA Hydration Solution. The purified DNA is ready for a variety of applications including restriction digestion, amplification, sequencing and membrane hybridization (Southern and dot blots).
Features and Benefits
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DNA Purity: |
O.D 260/280 ≥1.8 |
|
DNA Size Range: |
>50 kb, typical ~100-200 kb |
|
DNA Yield: |
10-20 µg/ml overnight cluture |
|
Safe Handling: |
No Phenol/Chloroform extraction |
Performance
High Molecular Weight DNA purified from Bacteria Using EZ High MW Bacterial DNA Isolation Kit
1 2 3 M
Genomic DNA (Lanes 1-3) was isolated from Ecoli DH5α using EZ High MW Bacterial DNA Isolation Kit and analyzed on a 0.8% Agarose gel. M: 1Kb Ladder.
Kit Components
|
Components |
M1012-50 |
M1012-100 |
|
Cell Suspension Solution |
30 ml |
60 ml |
|
Nuclei Lysis Solution |
30 ml |
60 ml |
|
Protein Precipitation Solution |
10 ml |
20 ml |
|
DNA Hydration Solution |
10 ml |
20 ml |
|
RNaseA Solution |
0.15 ml |
0.3 ml |
|
Lysozyme |
1 tube |
2 tubes |
|
Lysozyme Re-suspension Solution |
3 ml |
3 ml x 2 | |
