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Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates for Bacterial Growth
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
 
EZ Eco-Direct Tissue/Tail PCR Genotyping Kit
 
EZ Eco-Direct Tissue/Tail PCR Genotyping Kit is designed for rapid extraction and amplification of genomic DNA from mouse tail/toe/ear punch and animal tissues. With the EZ Eco-Direct Tissue/Tail PCR Genotyping Kit the extraction of genomic DNA is done by heating the sample in Extraction Solution I at 95oC for 15 min and then mixed with Extraction Solution II prior to PCR. An aliquot of the DNA extraction is mixed with a 2X PCR Ready Mix III included in the kit and user-selected primers to amplify target DNA. The 2X PCR Ready Mix III containing buffer, dNTP and Taq polymerase is specially optimized for use with the extracted DNA. The 2X PCR Ready Mix III contains an inert red dye that allows direct loading of PCR products onto agarose gels to visualize amplified target. 
Features and Benefits      
Fast Protocol:
Multiplexing:
Significant savings:
Consistent results:
Genomic DNA ready for PCR in 15 min. No overnight digestion
Strip tube for parallel processing
Cost-effective, no need to purify genomic DNA with expensive spin columns.
Virtually 100% success rate!
Performance
 
 
 
 
 
PCR analysis of DNA extracted from mouse tails using EZ Eco-Direct Tissue/Tail PCR Genotyping Kit
Mouse genomic DNA was extracted from eight mouse tails and PCR amplification of the two target genes was carried out using EZ Eco-Direct Tissue/Tail PCR Genotyping Kit. The amplified DNA fragments (220 bp in lanes 1-8 and 498 bp in lanes 1-8) were analyzed on a 2% agarose gel. M: 100 bp DNA Even Ladder.
 
Kit Components
  
Components
ECO-100
ECO-500
ECO-1000
Extraction Solution I 
6 ml
30  ml
2 x 30 ml
Extraction Solution II 
6 ml
30  ml
2 x 30 ml
2X PCR Ready Mix III
1 ml
5 x 1 ml
10 x 1 ml
 

 

 

 

 

Products (Total Items: 6)

ECO-100 EZ Eco-Direct Tissue/Tail PCR Genotyping Kit 100 reactions $55.00
ECO-500 EZ Eco-Direct Tissue/Tail PCR Genotyping Kit 500 reactions $175.00
ECO-1000 EZ Eco-Direct Tissue/Tail PCR Genotyping Kit 1000 reactions $340.00
P4000-100 2X PCR Ready Mix III for Genotyping 100 reactions $46.00
P4000-500 2X PCR Ready Mix III for Genotyping 500 reactions $163.00
P4000-1000 2X PCR Ready Mix III for Genotyping 1000 reactions $305.00

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