|
The EZ Tissue/Tail DNA Isolation Kit is designed for isolating genomic DNA from animal tissues including rodent tails. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Tissue lysate is prepared by digesting tissues with proteinase K in a specially formulated lysis buffer that allows protein denaturation. The lysate is then mixed with binding buffer and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion and southern blotting. |
