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Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
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HF Taq DNA Polymerase
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2X Taq Master Mix
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dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates for Bacterial Growth
Pipette Tips
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Freezer Boxes
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Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
 
EZ Blood/Cell DNA Isolation Kit
 
EZ Blood/Cell DNA Isolation Kit is designed for isolating genomic DNA from whole blood or cells. The kit is based on selective binding of DNA to silica membrane in the presence of chaotropic salts. Blood or cell lysate is prepared by digesting cells with Proteinase K in a specially formulated lysis/binding buffer that allows protein denaturation. RNA is removed by RNase digestion. The lysate is then mixed with ethanol and applied to the silica membrane-based spin column. Impurities are removed by washing steps. Genomic DNA is eluted in low salt elution buffer. The purified DNA is suitable for PCR, restriction enzyme digestion, southern blotting and other molecular biology applications. 
 
Features and Benefits
 
DNA Purity:
DNA Size Range:

DNA Yield:

Spin Column Binding Capacity:

Elution Volume:

Typical O.D 260/280 =1.8, free of RNA
>20 kb
5-15 g
50 g DNA
200 l
 
 
Performance
 
PCR-ready high quality DNA purified using EZ Blood/Cell DNA Isolation Kit 
A                                                                                                       B
    M       1        2       3        4        5        6                    M      1       2        
                    
A. Genomic DNA was purified from blood (lanes 1-4) and cells (lanes 5-6) using EZ Blood/Cell DNA Isolation Kit and analyzed on 1% agarose gel. M: Lambda-HindIII Ladder. B. PCR amplification was performed on DNA isolated from blood. Lane 1: 498 bp amplicon from AP2 gene. Lane 2: 881 bp amplicon from MVP gene. M: 100 bp ladder.
 
Kit Components
 

Components

M1004-50

M1004-250

Lysis/Binding Buffer

13 ml

55 ml

Wash Buffer I

15 ml

62.5 ml

Wash Buffer II

9 ml

37.5 ml

Elution Buffer

25 ml

110 ml

Proteinase K

1 ml

5 ml

RNaseA

1 ml

5 ml

Spin Column Units

50

250

Sample Collection Tubes

50

250

Products (Total Items: 2)

M1004-50 EZ Blood/Cell DNA Isolation Kit 50 preps $87.00
M1004-250 EZ Blood/Cell DNA Isolation Kit 250 preps $360.00

Copyright 2006 EZ BioResearch LLC LLC. All Rights Reserved.