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Tissue/Tail PCR Genotyping
SV Tissue/Tail Genotyping
Eco-Direct Tissue/Tail Genotyping
Blood/Cell PCR Genotyping
Zebrafish PCR Genotyping
Tissue/Tail DNA + PCR Kit
High MW Tail DNA + PCR Kit
Taq DNA Polymerase
Turbo Taq DNA Polymerase
HotStart Taq DNA Polymerase
HF Taq DNA Polymerase
Long Taq DNA Polymerase
2X Taq Master Mix
2X Taq Red Master Mix
2X Turbo Taq Master Mix
2X Turbo Taq Red Master Mix
dNTP Mix
Tissue/Tail DNA Isolation
Blood/Cells DNA Isolation
High MW Tissue DNA Isolation
High MW Blood DNA Isolation
High MW Tail DNA Isolation
High MW Cell DNA Isolation
High MW Bacterial DNA Isolation
Plasmid Miniprep
High Speed Plasmid Maxiprep
Plasmid Maxiprep
PCR Clean-Up
Gel Extraction
Tissue/cell RNA Mini Kit
Tissue/cell RNA Mini Plus Kit
RNA Clean-Up
RNA Clean-Up Plus DNase Kit
EZ Blood Total RNA Isolation
Stabilized Blood RNA Isolation
EZ RNA Storage Solution
EZ MicroElute-10 DNA Clean-Up Kit
EZ MicroElute-25 DNA Clean-Up Kit
EZ DNA MW Markers
EZ Protein MW Markers
Water and Reagents
Agar Plates for Bacterial Growth
Pipette Tips
PCR Tubes
Microcentrifuge Tubes
Centrifuge Tubes
Culture Tubes
T-shape Spreaders
Inoculating Needles
Petri Dishes
Freezer Boxes
Plastic Freezer Boxes
Cryogenic Vials
Cell Culture Plates
Cell Culture Flasks
Cell Culture Chamber Slides
Cell Culture Dishes
Cell Scrapers and Lifters
Serological Pipettes
Cell Strainer
Science Kits
 
EZ Fast Blood/Cell PCR Genotyping Kit
 
EZ Fast Blood/Cell PCR Genotyping Kit is designed for rapid extraction and amplification of genomic DNA from whole blood, blood dried on a blood card and cultured mammalian cells without DNA purification. With EZ Fast Blood/Cell PCR Genotyping Kit the extraction of genomic DNA is a simple fast single-tube procedure. Briefly, the DNA is released from the blood or cells to the Blood Lysis Solution by 5-15 minute incubation of the sample with the Blood Lysis Solution. There is no need for organic extraction and precipitation of the DNA. After neutralization, the DNA extraction is ready for PCR. An aliquot of the DNA extraction is mixed with a 2X PCR Ready Mix included in the kit and user-selected primers to amplify target DNA. The 2X PCR Ready Mix containing buffer, dNTP and Taq polymerase is specially optimized for use with the extracted DNA. The 2X PCR Ready Mix contains an inert red dye that allows direct loading of PCR products onto agarose gels to visualize amplified target.
 
Features and Benefits
Fast extraction:
Easy simple protocol:
Significant savings:
Safe Handling:
Consistent Result:
15 min extraction of genomic DNA
Single tube and little tube handling
No expensive spin columns
No Phenol/Chloroform extraction
Extremely high success rate
 
Performance
 
PCR analysis of DNA extracted from blood samples using EZ Fast Blood/Cell PCR Genotyping Kit
 
    M      1      2       3      4       5       6
DNA was extracted from 10 ul of whole blood in 5 minutes and PCR amplification of the target genes was carried out using the EZ Fast Blood/Cell PCR Genotyping Kit. Amplified DNA fragments (220 bp in lanes 1-2, 498 bp in lanes 3-4 and 938 bp in lanes 5-6) were analyzed on a 2% agarose gel. M: 100 bp ladder.
 
Kit Components

Components

G1002-10

G1002-100

G1002-300

Blood/Cell Lysis Solution

200 ul

2 ml

6 ml

Blood/Cell Neutralization Solution

2 ml

20 ml

60 ml

2X PCR Ready Mix

100 ul

1 ml

3 ml

Products (Total Items: 6)

G1002-10 Fast Blood/Cell PCR Genotyping Kit (trial) 10 reactions $19.95
G1002-100 Fast Blood/Cell PCR Genotyping Kit 100 reactions $139.00
G1002-300 Fast Blood/Cell PCR Genotyping Kit 300 reactions $389.00
P1000-100 2X PCR Ready Mix For Genotyping 100 reactions $56.00
P1000-300 2X PCR Ready Mix For Genotyping 300 reactions, 50% discount shipping $163.00
P1000-1000 2X PCR Ready Mix For Genotyping 1000 reactions, Free Shipping $532.00

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